The assessment of targeted and non-targeted genome damages in human somatic cells using the combination of cytogenetic and comet assay data (2023) ConRad 2023, “Global Conference on Radiation Topics - Preparedness, Response, Protection and Research” (25th Nuclear Medical Defence Conference), Munich, Germany 2023 .
Prof. Dr. Iryna Smetanska
Lycium schweinfurthii is a traditional medicinal plant grown in the Mediterranean region. As it is used in folk medicine to treat stomach ulcers, it took more attention as a source of valuable secondary metabolites. The in vitro cultures of L. schweinfurthii could be a great tool to produce secondary metabolites at low costs. The presented study aimed to introduce and optimize a protocol for inducing callus and cell suspension cultures as well as estimating phenolic, flavonoid compounds, and antioxidant activity in the cultures of the studied species. Three plant growth regulators (PGRs) were supplemented to MS medium solely or in combination to induce callus from leaf explants. The combination between 2,4-dichlorophenoxy acetic acid (2,4-D) and 1-naphthyl acetic acid (NAA) induced callus in all explants regardless of the concentration. The highest fresh weight of callus (3.92 g) was obtained on MS medium fortified with 1 mg L−1 of both 2,4-D and NAA (DN1) after 7 weeks of culture. DN1 was the best medium for callus multiplication regarding the increase in fresh weight and size of callus. Otherwise, the highest phenolics, flavonoids, and antioxidant activity against DPPH free radicals were of callus on MS fortified with 2 mg L−1 NAA (N2). The cell suspension cultures were cultivated on a liquid N2 medium with different sucrose concentrations of 5–30 g L−1 to observe the possible effects on cells’ multiplication and secondary metabolite production. The highest fresh and viable biomass of 12.01 g was obtained on N2 containing 30 g L−1 sucrose. On the other hand, the cell cultures on N2 medium of 5 and 30 g L−1 sucrose produced phenolics and flavonoids, and revealed
In recent decades, there has been a huge level of interest in bioactive compounds fromnatural sources. The large range of plant biodiversity means that there is a huge varietyof bioactive compounds. Bioactive compounds are mostly specific secondary metaboliteswith antioxidant, inflammatory, immunomodulative potential, antimicrobial properties, etc.The bioactive compounds of classes of terpenes, flavonoids, alkaloids, coumarins, stilbenes,etc., alongside the description of some of their mechanisms of action, are important tostudy. It is important to note that the use of plant extracts complicates the identificationof the effects of antiviral, antimicrobial or other capacities of separate biologically activecompounds; additionally, the effects of activities of solute compounds in extracts can causean additive (the synergistic effect) or antogonistic effect to come into play. In that context,the current issue is open for scientific research on the description of novel isolated bioactivecompounds, as well as some of their unknown effects, which are a priority for the appliedneeds of humans in different areas of life.
M.Sc. Diaa Mohammed,
Dr. Ahmed M. M. Gabr,
Prof. Dr. Iryna Smetanska
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