Prof. Dr. Michael Schrader

Physikalische Chemie und Instrumentelle Analytik

Adresse: A3.505
Am Hofgarten 10
85354 Freising
Funktionen: Beauftragter für internat. Beziehungen in den Studiengängen BT und MBT (seit 2015)
Prodekan von 2021 bis 2023 (Dekan 2015 - 2021)
Fakultät: Fakultät Bioingenieurwissenschaften

Sprechstunde: donnerstags 10:30 bis 11 Uhr oder nach Absprache, auch per Zoom.

Lehrveranstaltungen
Physikalische Chemie, mehrere Module (B.Sc.), eigenes Lehrbuch

Instrumentelle Bioanalytik, mehrere Module (B.Sc.)

Peptid- und Protein-Analytik sowie Protein Science (M.Sc.), Herausgeber Methodenbuch

Bachelor- und Masterarbeiten in obigen Bereichen, eigene Anleitung

modulverantwortlich bei GMP (B.Sc.) und Rechtl. Grundl. (M.Sc.)
Persönliches Leitbild meiner Lehre
VITAM IMPENDERE VERO (Motto meiner Alma mater; Juvenal: Das Leben der Wahrheit gewidmet)
sowie MASS UND MITTE BEWAHREN (gemäß Konfutse, Lun Yu - Gespräche)

Folgende Aspekte sind für mich Motivation und Kompass meines Handelns:

Ausbilden auf dem in Industrie und Forschungseinrichtungen benötigtem anspruchsvollen Niveau
von Fach- und persönlichen Kompetenzen in interdisziplinärem Bezug zur Biotechnologie

Orientieren am Ziel maximal erreichbarer Anzahl und Zufriedenheit von Absolvent:innen
solcher Qualität, unter Schonung der notwendigen Ressourcen

Vielseitiges Fordern und gezieltes individuelles Fördern unserer Studierenden,
gemäß Ihrer Talente und Bereitschaft; dazu Transparenz mit klaren Einschätzungen,
unabhängig von Geschlecht und Herkunft - bei respektvollem Umgang miteinander.

Offenes und konstruktives Zusammenarbeiten mit meinen Kolleg:innen sowie externen Partnern,
geleitet von der Vorgabe, den maximalen Nutzen zu obigen Zielen gemeinsam zu erreichen

Nutzbringendes und dabei achtsames wie nachhaltiges Einsetzen meiner Energie,
bei kontinuierlicher Optimierung

Was motiviert mich dabei:

Hingabe für exakte Naturwissenschaften und durch Werte gelenkte Anwendung dieses Wissens

Begeisterung für den Campus Weihenstephan (Know-how, Vielfalt und Lage)

Nutzung der vielfältigen Möglichkeiten und Kontakte in der Bioregion München, wie auch darüber hinaus
Gastvorträge und -vorlesungen
Hochschulen im Ausland: Univ. Pécs (HU), Univ. Havanna (CU), FHNW Muttenz (CH), IUT Thionville (FR)

Hochschulen im Inland: HS Anhalt, HS Mannheim und HS Zittau/Görlitz

Konferenzen: Humboldt-Kollegs 2019 (Ibarra, EC) und 2012 (Havanna, CU)

PublicClimateSchool in München 2020 und 2019 (2020: https://youtu.be/ofDeihIB4m0)

Publikationen

Zeitschriftenbeiträge (peer-reviewed)
- Harald John, Tamara Lindl, Henrik Reuter, Wolfgang Schmeißer, Prof. Dr. Michael Schrader, Horst Thiermann
  
  Berechtigungen: Open Access
  
  Berechtigungen: Peer Reviewed
  
  Phosphonylated tyrosine and lysine residues as biomarkers of local exposure of human hair to the organophosphorus nerve agents sarin and VX (2023) Drug Testing and Analysis 15 (7), S. 730-744. DOI: 10.1002/dta.3459
  
  Mehr
- Harald John, Philipp Hörmann, Prof. Dr. Michael Schrader, Horst Thiermann
  
  Berechtigungen: Open Access
  
  Berechtigungen: Peer Reviewed
  
  Alkylated glutamic acid and histidine derived from protein-adducts indicate exposure to sulfur mustard in avian serum (2022) Drug Testing and Analysis 14 (6), S. 1140-1148. DOI: 10.1002/dta.3236
  
  Sulfur mustard (SM, bis[2-chloroethyl]-sulfide) is a banned chemical warfare agent deployed in the violent conflict in the Middle East poisoning humans and animals. For legal reasons, bioanalytical methods are mandatory proving exposure to SM. Reaction products (adducts) of SM with endogenous proteins, for example, serum albumin (SA), are valuable long-lived targets for analysis. Whereas nearly all methods known so far focus on human proteins, we address for the first time neat chicken SA and avian serum from chicken, duck, and ostrich. After proteolysis, protein precipitation, evaporation of the supernatant, and re-dissolution analysis were performed by micro-liquid chromatography-electrospray ionization tandem-mass spectrometry in the selected reaction monitoring mode, μLC-ESI MS/MS (SRM), for detection of the hydroxyethylthioethyl product ion [HETE]+ at m/z 105.0. After in vitro incubation with SM and pronase-catalyzed proteolysis, the alkylated amino acids Glu(-HETE) and His(-HETE) were detected. Both borne the SM-characteristic HETE-moiety bound to their side chain. The eightfold deuterated SM analog (d8-SM) was also applied to support adduct identification. Proteolysis conditions were optimized with respect to pH (8.0), temperature (50°C), and time to maximize the yield of Glu(-HETE) (30 min) and His(-HETE) (180 min). Amino acid adducts were stable in the autosampler for at least 24 h. Protein-adducts were stable in serum at −30°C for at least 33 days and for three freeze-and-thaw cycles. At the body temperature of chicken (+40°C), Glu(-HETE) was degraded in serum (period of half-change 3 days), whereas His(-HETE) remained stable. The presented method broadens the toolbox of procedures to document poisoning with SM.
  Mehr
- Prof. Dr. Michael Schrader, Peter Schulz-Knappe, Lloyd Fricker
  
  Berechtigungen: Open Access
  
  Berechtigungen: Peer Reviewed
  
  Historical perspective of peptidomics (2014) EuPA Open Proteomics 3 , S. 171-182. DOI: 10.1016/j.euprot.2014.02.014
- Michael Hamacher, al. et, Prof. Dr. Michael Schrader, al. et, Helmut E. Meyer
  
  Berechtigungen: Peer Reviewed
  
  HUPO Brain Proteome Project: Summary of the pilot phase and introduction of a comprehensive data reprocessing strategy (2006) Proteomics 2006 , S. 4890-8. DOI: 10.1002/pmic.200600295
- Prof. Dr. Michael Schrader, Hartmut Selle
  
  Berechtigungen: Peer Reviewed
  
  The process chain for peptidomic biomarker discovery (2006) Disease Markers . DOI: 10.1155/2006/174849
- Hartmut Selle, al. et, Prof. Dr. Michael Schrader, Hans-Dieter Zucht
  
  Berechtigungen: Peer Reviewed
  
  Identification of novel biomarker candidates by differential peptidomics analysis of cerebrospinal fluid in Alzheimer's disease (2005) Combinatorial Chemistry & High Throughput Screening 2005 , S. 801 - 806. DOI: 10.2174/138620705774962391
- Thomas Möhring, Markus Kellmann, Michael Jürgens, Prof. Dr. Michael Schrader
  
  Berechtigungen: Peer Reviewed
  
  Top-down identification of endogenous peptides up to 9 kDa in cerebrospinal fluid and brain tissue by nanoelectrospray quadrupole TOF tandem MS (2005) DOI: 10.1002/jms.741
- Prof. Dr. Michael Schrader, Peter Schulz-Knappe
  
  Berechtigungen: Peer Reviewed
  
  Peptidomics technologies for human body fluids (2001) Trends Biotechnol 2001 , S. S55-60. DOI: 10.1016/S0167-7799(01)01800-5
- Susanne Neitz, Prof. Dr. Michael Schrader, al. et
  
  Berechtigungen: Peer Reviewed
  
  Screening for disulfide-rich peptides in biological sources by carboxyamidomethylation in combination with differential matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (2001) Rapid Commun Mass Spectrom 2001 , S. 1586-1592. DOI: 10.1002/rcm.413
- Peter Schulz-Knappe, Hans-Dieter Zucht, al. et, Prof. Dr. Michael Schrader
  
  Berechtigungen: Peer Reviewed
  
  Peptidomics The Comprehensive Analysis of Peptides in Complex Biological Mixtures (2001) Combinatorial Chemistry & High Throughput Screening 2001 , S. 207 - 217. DOI: 10.2174/1386207013331246
- Rudolf Richter, Peter Schulz-Knappe, Prof. Dr. Michael Schrader, al. et, Wolf-Georg Forssmann
  
  Berechtigungen: Peer Reviewed
  
  Composition of the peptide fraction in human blood plasma: database of circulating human peptides (1999) J Chromatogr B Biomed Sci Appl 1999 , S. 25-35. DOI: 10.1016/S0378-4347(99)00012-2
- Volker Badock, Manfred Raida, al. et, Prof. Dr. Michael Schrader
  
  Berechtigungen: Peer Reviewed
  
  Distinction between the three disulfide isomers of guanylin 99-115 by low-energy collision-induced dissociation (1998) Rapid Commun Mass Spectrom 1998 , S. 1952-6. DOI: 10.1002/(SICI)1097-0231(19981215)12:23<1952::AID-RCM420>3.0.CO;2-N
- Juan J. Calvete, Prof. Dr. Michael Schrader, Manfred Raida, al. et
  
  Berechtigungen: Open Access
  
  Berechtigungen: Peer Reviewed
  
  The disulphide bond pattern of bitistatin, a disintegrin isolated from the venom of the viper Bitis arietans (1997) FEBS Lett 1997 , S. 197-202. DOI: 10.1016/S0014-5793(97)01203-9
- Peter Schulz-Knappe, Prof. Dr. Michael Schrader, al. et, Wolf-Georg Forssmann
  
  Berechtigungen: Peer Reviewed
  
  Peptide bank generated by large-scale preparation of circulating human peptides (1997) Journal of Chromatography A 1997 , S. 125-132. DOI: 10.1016/S0021-9673(97)00152-0
- Prof. Dr. Michael Schrader, Michael Jürgens, al. et, Wolf-Georg Forssmann
  
  Berechtigungen: Peer Reviewed
  
  Matrix-assisted laser desorption/ionisation mass spectrometry guided purification of human guanylin from blood ultrafiltrate (1997) Journal of Chromatography A 1997 , S. 139-145. DOI: 10.1016/S0021-9673(97)00169-6
Bücher / Monografien
- Prof. Dr. Michael Schrader
  
  Sicher zur Abschlussarbeit
  in Natur- und Ingenieurwissenschaften (2022) Sicher zur Abschlussarbeit, in Natur- und Ingenieurwissenschaften, 1. Aufl. Springer Spektrum, Wiesbaden . DOI: 10.1007/978-3-658-36544-8
- Prof. Dr. Michael Schrader, Lloyd Fricker
  
  Peptidomics - Methods and Strategies (2018) Humana Press, New York, 1. Auflage 2018, 423 S., 78 Abbildungen . DOI: 10.1007/978-1-4939-7537-2
  
  This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls. Authoritative and practical, Peptidomics: Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics.
- Prof. Dr. Michael Schrader
  
  Prinzipien und Anwendungen der Physikalischen Chemie (2016) Springer Spektrum, Springer-Verlag Berlin Heidelberg, 1. Auflage 2016, 327 S., 56 schwarz-weiß Abbildungen . DOI: 10.1007/978-3-642-41730-6
Beiträge in Monografien, Sammelwerken, Schriftenreihen
- Prof. Dr. Michael Schrader
  
  Berechtigungen: Peer Reviewed
  
  Origins, Technological Development, and Applications of Peptidomics (2018) Methods in Molecular Biology 1719 , S. 3-39. DOI: 10.1007/978-1-4939-7537-2_1
- Prof. Dr. Michael Schrader, Petra Budde, al. et, Hans-Dieter Zucht
  
  Berechtigungen: Peer Reviewed
  
  Peptidomics Technologies and Applications in Drug Research (2006) Proteomics in Drug Research, Methods and Principles in Medicinal Chemistry . DOI: 10.1002/3527608230.ch6

Zurück zur Übersicht

Prof. Dr. Michael Schrader

Lehrveranstaltungen

Persönliches Leitbild meiner Lehre

Gastvorträge und -vorlesungen

Publikationen

Zeitschriftenbeiträge (peer-reviewed)

Phosphonylated tyrosine and lysine residues as biomarkers of local exposure of human hair to the organophosphorus nerve agents sarin and VX (2023) Drug Testing and Analysis 15 (7), S. 730-744. DOI: 10.1002/dta.3459

Alkylated glutamic acid and histidine derived from protein-adducts indicate exposure to sulfur mustard in avian serum (2022) Drug Testing and Analysis 14 (6), S. 1140-1148. DOI: 10.1002/dta.3236

Historical perspective of peptidomics (2014) EuPA Open Proteomics 3 , S. 171-182. DOI: 10.1016/j.euprot.2014.02.014

HUPO Brain Proteome Project: Summary of the pilot phase and introduction of a comprehensive data reprocessing strategy (2006) Proteomics 2006 , S. 4890-8. DOI: 10.1002/pmic.200600295

The process chain for peptidomic biomarker discovery (2006) Disease Markers . DOI: 10.1155/2006/174849

Identification of novel biomarker candidates by differential peptidomics analysis of cerebrospinal fluid in Alzheimer's disease (2005) Combinatorial Chemistry & High Throughput Screening 2005 , S. 801 - 806. DOI: 10.2174/138620705774962391

Top-down identification of endogenous peptides up to 9 kDa in cerebrospinal fluid and brain tissue by nanoelectrospray quadrupole TOF tandem MS (2005) DOI: 10.1002/jms.741

Peptidomics technologies for human body fluids (2001) Trends Biotechnol 2001 , S. S55-60. DOI: 10.1016/S0167-7799(01)01800-5

Screening for disulfide-rich peptides in biological sources by carboxyamidomethylation in combination with differential matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (2001) Rapid Commun Mass Spectrom 2001 , S. 1586-1592. DOI: 10.1002/rcm.413

Peptidomics The Comprehensive Analysis of Peptides in Complex Biological Mixtures (2001) Combinatorial Chemistry & High Throughput Screening 2001 , S. 207 - 217. DOI: 10.2174/1386207013331246

Composition of the peptide fraction in human blood plasma: database of circulating human peptides (1999) J Chromatogr B Biomed Sci Appl 1999 , S. 25-35. DOI: 10.1016/S0378-4347(99)00012-2

Distinction between the three disulfide isomers of guanylin 99-115 by low-energy collision-induced dissociation (1998) Rapid Commun Mass Spectrom 1998 , S. 1952-6. DOI: 10.1002/(SICI)1097-0231(19981215)12:23<1952::AID-RCM420>3.0.CO;2-N

The disulphide bond pattern of bitistatin, a disintegrin isolated from the venom of the viper Bitis arietans (1997) FEBS Lett 1997 , S. 197-202. DOI: 10.1016/S0014-5793(97)01203-9

Peptide bank generated by large-scale preparation of circulating human peptides (1997) Journal of Chromatography A 1997 , S. 125-132. DOI: 10.1016/S0021-9673(97)00152-0

Matrix-assisted laser desorption/ionisation mass spectrometry guided purification of human guanylin from blood ultrafiltrate (1997) Journal of Chromatography A 1997 , S. 139-145. DOI: 10.1016/S0021-9673(97)00169-6

Bücher / Monografien

Sicher zur Abschlussarbeit
in Natur- und Ingenieurwissenschaften (2022) Sicher zur Abschlussarbeit, in Natur- und Ingenieurwissenschaften, 1. Aufl. Springer Spektrum, Wiesbaden . DOI: 10.1007/978-3-658-36544-8

Peptidomics - Methods and Strategies (2018) Humana Press, New York, 1. Auflage 2018, 423 S., 78 Abbildungen . DOI: 10.1007/978-1-4939-7537-2

Prinzipien und Anwendungen der Physikalischen Chemie (2016) Springer Spektrum, Springer-Verlag Berlin Heidelberg, 1. Auflage 2016, 327 S., 56 schwarz-weiß Abbildungen . DOI: 10.1007/978-3-642-41730-6

Beiträge in Monografien, Sammelwerken, Schriftenreihen

Origins, Technological Development, and Applications of Peptidomics (2018) Methods in Molecular Biology 1719 , S. 3-39. DOI: 10.1007/978-1-4939-7537-2_1

Peptidomics Technologies and Applications in Drug Research (2006) Proteomics in Drug Research, Methods and Principles in Medicinal Chemistry . DOI: 10.1002/3527608230.ch6