Prof. Dr. Jörg Kleiber

Biochemie und Gentechnologie

Adresse: A3.620
Am Hofgarten 10
85354 Freising
Funktionen: Studiendekan und Studiengangsleiter im Master Biotechnologie/Bioingenieurwesen
Fakultät: Fakultät Bioingenieurwissenschaften

Telefonische Sprechstunde: Montag 9 bis 10 Uhr unter Tel.: 0157 73 77 68 09
Ersatzbüro wegen Bauarbeiten: A3.211

Lehrveranstaltungen
Biochemie

Gentechnik

Bioanalytik

Pharmazeutische Biotechnologie
Mitgliedschaften
Gesellschaft für Biochemie und Molekularbiologie
Wissenschaftliche Schwerpunkte
Nukleinsäureanalytik und molekulare Diagnostik
Kurze CV
Biologie-Studium an der Universität Karlsruhe

1988: Doktorarbeit am Lehrstuhl für Biochemie, Universität Karlsruhe

1989-1990: Postdoc Fellow an der School of Medicine, Yale University (New Haven, Connecticut)

1990-2008: Verschiedene Führungspositionen bei Boehringer Mannheim und Roche Diagnostics in Forschung und Entwicklung sowie Strategie und Marketing

2002: Master of Management am St. Galler Management-Institut

Seit 2009: Professor für Biochemie und Gentechnik/Molekularbiologie an der Hochschule Weihenstephan-Triesdorf

2018: Forschungssemester bei AgResearch (Ruakura Research Center) in Hamilton, Neuseeland

Publikationen

Zeitschriftenbeiträge (peer-reviewed)
- Fabian Model, Neal Osborn, David Ahlquist, Robert Gruetzmann, Bela Molnar, Ferenc Sipos, Orsolya Galamb, Christian Pilarsky, Hans-Detlev Saeger, Zsolt Tulassay, Kari Hale, Suzanne Mooney, Joseph Lograsso, Peter Adorjan, Ralf Lesche, Andreas Dessauer, Prof. Dr. Jörg Kleiber, Bärbel Porstmann, Andrew Sledziewski, Catherine Lofton-Day
  
  Berechtigungen: Peer Reviewed
  
  Identification and validation of colorectal neoplasia-specific methylation markers for accurate classification of disease (2007) Molecular Cancer Research 5 (2), S. 153-163. DOI: 10.1158/1541-7786.mcr-06-0034
- Prof. Dr. Jörg Kleiber, Thomas Walter, Gerd Haberhausen, Sue Tsang, Reiner Babiel, Maurice Rosenstraus
  
  Berechtigungen: Peer Reviewed
  
  Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA (2000) The Journal of Molecular Diagnostics 2 (3), S. 158-166. DOI: 10.1016/S1525-1578(10)60632-0
  
  We developed a homogeneous format reverse transcription-polymerase chain reaction assay for quantitating hepatitis C virus (HCV) RNA based on the TaqMan principle, in which signal is generated by cleaving a target-specific probe during amplification. The test uses two probes, one specific for HCV and one specific for an internal control, containing fluorophores with different emission spectra. Titers are calculated in international units (IU)/ml by comparing the HCV signal generated by test samples to that generated by a set of external standards. Endpoint titration experiments demonstrated that samples containing 28 IU/ml give positive results 95% of the time. Based on these data, the limit of detection was set conservatively at 40 IU/ml. All HCV genotypes were amplified with equal efficiency and accurately quantitated: when equal quantities of RNA were tested, each genotype produced virtually identical fluorescent signals. The test exhibited a linear range extending from 64 to 4,180,000 IU/ml and excellent reproducibility, with coefficients of variation ranging from 21.6 to 30.4%, which implies that titers that differ by a factor of twofold (0.3 log10) are statistically significant (P = 0.005). The test did not react with other organisms likely to co-infect patients with hepatitis C and exhibited a specificity of 99% when evaluated on a set of samples from HCV seronegative blood donors. In interferon-treated patients, the patterns of viral load changes revealed by the TaqMan HCV quantitative test distinguished responders from nonresponders and responder-relapsers. These data indicate that the TaqMan quantitative HCV test provides an attractive alternative for measuring HCV viral load and should prove useful for prognosis and for monitoring the efficacy of antiviral treatments.
- Albert Geiger, Ane Lester, Prof. Dr. Jörg Kleiber, Henrik Ørum
  
  Berechtigungen: Peer Reviewed
  
  PNA Array Technology in Molecular Diagnostics (1998) Nucleosides and Nucleotides 17 (9-11), S. 1717-1724. DOI: 10.1080/07328319808004706
- Prof. Dr. Jörg Kleiber, Frantisek Kalousek, Mamta Swaroop, Leon E. Rosenberg
  
  Berechtigungen: Peer Reviewed
  
  The general mitochondrial matrix processing protease from rat liver: structural characterization of the catalytic subunit (1990) Proceedings of the National Academy of Sciences of the United States of America 87 (20), S. 7978-7982. DOI: 10.1073/pnas.87.20.7978
Patente
- Prof. Dr. Jörg Kleiber, Christine Markert-Hahn, Herbert Harttig
  
  Automatable method for preparing samples which can be universally applied (1999) WO 99/16781 .
- Prof. Dr. Jörg Kleiber, Karim Tabiti, Gregor Sagner
  
  Integrated procedure and system for amplification and detection of nucleic acids (1999) DE 19730359 .
- Prof. Dr. Jörg Kleiber, Albert Geiger, Henrik Ørum
  
  Sequence-specific detection of nucleic acids by PNA arrays (1996) WO1996027680A1 .
- Prof. Dr. Jörg Kleiber, Thomas Walter, Herbert Harttig
  
  Magnetic Pigment (1996) WO 96/41811 .
- Prof. Dr. Jörg Kleiber, Cortina Kaletta, Rüdiger Rüger, Christoph Kessler
  
  Detection of bacteria by nucleic acid amplification (1992) EP0501356A1 .

Zurück zur Übersicht

Prof. Dr. Jörg Kleiber

Lehrveranstaltungen

Mitgliedschaften

Wissenschaftliche Schwerpunkte

Kurze CV

Publikationen

Zeitschriftenbeiträge (peer-reviewed)

Identification and validation of colorectal neoplasia-specific methylation markers for accurate classification of disease (2007) Molecular Cancer Research 5 (2), S. 153-163. DOI: 10.1158/1541-7786.mcr-06-0034

Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA (2000) The Journal of Molecular Diagnostics 2 (3), S. 158-166. DOI: 10.1016/S1525-1578(10)60632-0

PNA Array Technology in Molecular Diagnostics (1998) Nucleosides and Nucleotides 17 (9-11), S. 1717-1724. DOI: 10.1080/07328319808004706

The general mitochondrial matrix processing protease from rat liver: structural characterization of the catalytic subunit (1990) Proceedings of the National Academy of Sciences of the United States of America 87 (20), S. 7978-7982. DOI: 10.1073/pnas.87.20.7978

Patente

Automatable method for preparing samples which can be universally applied (1999) WO 99/16781 .

Integrated procedure and system for amplification and detection of nucleic acids (1999) DE 19730359 .

Sequence-specific detection of nucleic acids by PNA arrays (1996) WO1996027680A1 .

Magnetic Pigment (1996) WO 96/41811 .

Detection of bacteria by nucleic acid amplification (1992) EP0501356A1 .